Registration info:
Fees: 654 euros TTC (545 euros HT)
Deadline: 31 May 2016
Registration - Closed
Payment (3 alternative ways):
- Bon de Commande (French participants only)
- Bank transfer
- By Credit Card --- Payment Guideline
Program:
Lecturers/Trainers:
- Andreas Engel (TU Delft) -
- Neil Thomson (University of Leeds) - Stick or Twist : Atomic force microscopy of DNA systems
- Jean-Luc Pellequer (IBS, Grenoble) - Atomic Force Microscopy and Integrated Structural Biology
- Alessandro Podestà (University of Milano) - Characterizing nanoscale entities and interactions from AFM images
- Daniel Navajas (University of Barcelona) - Cell and tissue mechanics probed with Atomic Force Microscopy
- Frank Lafont (Institut Pasteur, Lille) - Correlative microscopy using AFM to study cell biomechanics
- Laura Picas (CBS, Montpellier) - Quantitative mapping of biological membranes: from in vitro to cellular membranes
- Filipp Oesterhelt (University of Tubingen) -
- Ignacio Casuso (U1006, INSERM, Marseille) - High Speed Atomic Force Microscopy: Why, How and Achievements
- Felix Rico (U1006, INSERM, Marseille) - Mechanics of single proteins
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- Simone Bovio (Institut Pasteur, Lille)
- Michaël Odorico (CEA, Marcoule)
- Laura Picas (CBS, Montpellier)
- Nicolas Buzhinsky (U1006, INSERM, Marseille)
- Felix Rico (U1006, INSERM, Marseille)
- Ignacio Casuso (U1006, INSERM, Marseille)
Practical Training:
Session: single molecule Force Spectroscopy
This session has for objective familiarizing the participant with the scientific and technical ´must-knows´ that are key for performing single molecule Force Spectroscopy measurements by AFM in a quantitative manner.
Host: Felix Rico (Lab for Biological AFM, U1006, Inserm, Marseille, France)
sample: Titin
machine: JPK NanoWizard 3
Session: High Speed AFM
In this session, the potential of High Speed AFM imaging for the visualization of biomolecular dynamics at high resolution will be shown, samples and probes will be prepared for high resolution, maximal imaging speeds will be searched providing technical details.
Host: Ignacio Casuso (Lab for Biological AFM, U1006, Inserm, Marseille, France)
Sample: Outer Membrane Porin F (OmpF)
Machine: High Speed AFM of the Ando´s type
Session: Cell Mechanics
The metabolic activity of living cells is intimately correlated with their mechanical properties. The AFM is an empowering tool for the study of cell mechanics, in this session details and know-how tips on performing successful AFM cell mechanics are provided.
Host: Simone Bovio (Institut de Biologie de Lille, CMIP, Lille, France)
Sample: Fibroblast cells
Machine: BioScope Cathalyst AFM integrated on Olympus IX71 light microscope, Bruker
Session: Conventional biomolecule AFM
This session is a technical hands-on practice and comparison of well-established methodologies in the visualization of biomolecules by AFM. Different modes of operation (Contact Mode, Tapping Mode and Peak Force Mode) will be mutually compared to provide an overview of different possible techniques available for the visualization of biomolecules by AFM.
Hosts: Nicolas Buzhinsky (Lab for Biological AFM, U1006, Inserm, Marseille, France)
Laura Picas (Centre de Biochimie Structurale de Montpellier, Montpellier, France)
Sample: Bacteriorhodopsin; Supported Lipid Bilayer presenting multiple lipid phases
Machine: Multimode V equipped of a Nanoscope V controller, Bruker
Local Organizers:
- Simon Scheuring
- Felix Rico
- Ignacio Casuso
- Sophie Gall
- Nicolas Buzhinsky
Venue:
Meeting site: Luminy Campus, Marseille 13009 - Campus Plan (pdf)
Public transport: Bus connection between the Airport and the St Charles Train Station. At the train station take the Metro line 2, stop at
"Rond Point du Prado". Then take the bus N21 till Luminy (terminus).
Marseille public transport: www.rtm.fr
Accommodation: Cité Universitaire/Studinettes, Bât. B - Jul.18 - Jul.23; 5 nights covered by the inscription fees.
Alimentation: University SOGERES cantine - Two meals per day (lunch and dinner)
ATTENDEES at WORK
