AFM BioMed Conference

 

 Special Keynote Lecture (Online)

 

   "Future of Scientific Conference"

   Cyrus ModyMaastricht University, Netherland 

  

 

 Keynote Lecture

 

 Simon ScheuringWeill Cornell Medicine, USA 

Simon Scheuring is Professor of Physiology and Biophysics in Anesthesiology at Weill Cornell Medicine, New York, USA. He is a trained biologist from the Biozentrum at the University of Basel, Switzerland (1992−1996). During his M.Sc. and Ph.D. (1996−2001; Andreas Engel laboratory), he learned electron microscopy and atomic force microscopy, and got interested in membrane proteins, working on the structure determination of aquaporins and sugar transporters. During his postdoc (2001−2004; Jean-Louis Rigaud laboratory) and research assistant (2004−2007) at the institut national de la santé et de la recherche médicale (INSERM) and the Institut Curie in Paris, France, he learned membrane physical chemistry and developed atomic force microscopy for the study of native membranes. As junior research director (2007−2012), he set up his independent lab at the Institut Curie in Paris, France. Next, promoted to senior research director, he built a larger laboratory at INSERM/Aix-Marseille Université in Marseille, France, (2012-2016). He then moved to Weill Cornell Medicine, New York, USA (2017), where he got appointed as Professor of Physiology and Biophysics in Anesthesiology. Simon Scheuring’s laboratory is specialized in Atomic Force Microscopy (AFM) based technologies for the study of various membrane phenomena, such as membrane protein structure, assembly, diffusion and conformational dynamics. Over the past years, his laboratory has been instrumental in the development and application of High-Speed Atomic Force Microscopy (HS-AFM), unique for the analysis of dynamics of unlabeled single molecules, allowing to bridge structure and function. To make HS-AFM more powerful for biological applications, his laboratory developed environmental control, i.e. slow and fast buffer exchange, temperature and force control. Further developments concerned novel AFM-based modalities, by integrating optical microscopy into a HS-AFM, and by developing high-speed force spectroscopy (HS-FS) and high frequency microrheology (HF-µR). The laboratory also developed HS-AFM line scanning (HS-AFM-LS) and HS-AFM height spectroscopy (HS-AFM-HS) which allow to probe molecular dynamicsat millisecond and microsecond temporal resolution. Most recently, Simon Scheuring’s laboratory introduced Localization Atomic Force Microscopy (LAFM) a super-resolution method that allows the extraction of quasi-atomic structural details from single molecule AFM data. Taking advantage of these possibilities, Simon Scheuring made significant contributions in the membrane trafficking and the channels & transporters fields. Simon Scheuring has received several awards, most recently the NIH director's pioneer award (2019).

 

 

 Hirofumi Yamada, Kyoto University, Japan

 


 

Chair Persons & Invited Speakers

 

     Session: Imaging

           

        chair person:  Alice Pyne, The University of Sheffield, UK 

 

Dr Alice L. B. Pyne is an Associate Professor in Polymers and Soft Matter in the Department of Materials Science and Engineering at the University of Sheffield, and is head of the Royce Nanocharacterisation laboratory. Her research combines high-resolution atomic force microscopy (AFM) and image analysis to determine how the structural and conformational heterogeneity in individual DNA molecules affect fundamental biological processes. Alice received an MSci in Physics from the University of Bristol in 2009, and an EngD in biophysics from the London Centre for Nanotechnology, University College London, in 2015. She was awarded an EPSRC Doctoral Prize Fellowship, followed by an MRC Rutherford Innovation Fellowship, to establish an independent research programme in high resolution biomolecular AFM. Throughout her career, Alice has worked closely with industry to develop novel AFM methods capable of routinely resolving the DNA double helix on individual molecules. She has pioneered these methods to achieve time-resolved imaging of DNA at the sub-molecular scale, showing DNA molecules twisting and “dancing” in ways that had not previously been accessible. Alice is building a community in the biosciences that adopts her high-resolution imaging methods, enabled by developing an automated image analysis pipeline TopoStats which combines AFM image correction, molecule identification, and tracing into a single tool. She is spearheading an international effort to promote quantitative tools for analysis in AFM (www.github.com/AFM-SPM).

      

        invited speaker:  Roderick Lim, University of Basel, Switzerland       

 

     Session: Force

                       

        chair person:   Felix Rico, Aix-Marseille University & INSERM & CNRS, France

 

Felix Rico is associate professor in the department of Physics of Aix-Marseille University since 2013 and researcher at the LAI U1067, a joint Aix-Marseille University, CNRS & INSERM laboratory. He studied Physics at the University Autonoma of Barcelona and received his PhD in Biophysics from the University of Barcelona. He was postdoc at the University of Miami Miller School of Medicine (Miami, FL) and then at Institute Curie (Paris). He has been working in force microscopy since 2001, focusing on the mechanics and adhesion properties of biological systems. He has developed various force spectroscopy-based approaches to investigate the mechanics of single biomolecules, membranes and cells. He pioneered high-speed force spectroscopy to probe the mechanics and dynamics of single biomolecules and living cells at microsecond timescales.

            

        invited speaker:  Thomas Perkins, JILA/University of Colorado Boulder, USA 

 

Thomas Perkins is a Fellow of JILA, a joint institute between the US National Institute of Standards and Technology (NIST) and the University of Colorado. He graduated from Harvard University, did his graduate work at Stanford under Steve Chu, and post-doctoral studies at Princeton and Stanford with Steve Block. His group specializes in developing and applying high-precision single-molecule techniques to answer biological questions. Over the past decade, his group developed a series of focused-ion-beam modified cantilevers to study the folding and unfolding of proteins with enhanced precision and stability. By using cantilevers optimized for 1-µs resolution, his group re-examined the unfolding of individual bacteriorhodopsin (bR) molecules in native lipid bilayers with a 100-fold higher time resolution and a 10-fold higher force precision than previous studies. The unfolding pathway was resolved with unprecedented detail, indicating the structural elements associated with bR unfolding that were as small as two amino acids. These advances in AFM metrology have been merged with site-specific conjugation to investigate the dynamics and energetics of a wide range of additional systems ranging from small globular proteins and structured RNA molecules to effector proteins and cardiac myosin. Tom is a Fellow of the American Association for the Advancement of Science and the American Physical Society. He is also a long-time co-organizer of the biennial Single Molecule Biophysics meeting at the Aspen Center for Physics. Tom has received a number of awards, including the Dept. of Commerce’s Gold Medal (its highest award) and its Ron Brown Excellence in Innovation Award, an Arthur S. Flemming Award for outstanding achievement in government service, and the Colorado Governor’s award for high impact research.

 

 

     Session: Cellular & Tissue Mechanics

                       

        chair person:  Yuri KorchevImperial College London, UK  

 

Yuri Korchev is a professor of biophysics at Imperial College London, Faculty of Medicine, UK since 2005 and a Professor at the Nano Life Science Institute (WPI-NanoLSI) in the Kanazawa University. For more than a decade, he was thrilled by the enormous potential of scanning ion conductance microscopy (SICM) for studies of cell physiology and biophysics. He was the first to image live cells with SICM in 1997. Since then, his group has made numerous technological developments for studying various aspects of live cell function on a nanoscale. His research span on development and applications of SICM for biomedical research. These developments sparked worldwide interest to SICM, establishing a distinct sub-field in the nanoscience and nanotechnology.

            

        invited speaker:  Takaharu OKAJIMA, Hokkaido University, Japan 

 

 

Takaharu Okajima is a Professor at Hokkaido University, Faculty of Information Science and Technology since 2013. He received his Ph.D. in Physics from Tokyo Institute of Technology in 2000. He joined the Department of Bioscience and Biotechnology at Tokyo Institute of Technology as an assistant professor (1995-2003). Since 2000, he started to develop AFM techniques for measuring mechanical properties of biological samples in the laboratory of Prof. Atsushi Ikai at Tokyo Institute of Technology and then in the laboratory of Prof. Hiroshi Tokumoto in the Nanotechnology Research Center at Hokkaido University. After he moved to the Graduate School of Information Science and Technology at Hokkaido University in 2007, he focused on his research in investigating the universal and specific features of cell rheological properties with AFM. A current focus of his work with AFM is on exploring physical mechanism of multicellular systems such as developing embryo at the single cell level.

 

            

     Session: Technology & Theory

                       

        chair person:   Noriyuki Kodera, Kanazawa University, Japan

 

Noriyuki Kodera is a Professor at the Nano Life Science Institute (WPI-NanoLSI) in the Kanazawa University. He studied Physics and received his PhD from Kanazawa University in 2005, where he was involved in the development of high-speed AFM (HS-AFM) at the laboratory of Prof. Toshio Ando. During his postdoc at Kanazawa University (2005-2010), he began research in biological application studies using HS-AFM and succeeded in the direct observation of myosin V walking along actin filaments. In 2010, he joined the faculty at Kanazawa University. After that, he has been continuously developing and improving the methods related to HS-AFM and observing proteins at work, particularly cytoskeleton-related proteins and intrinsically disordered proteins, using HS-AFM for providing novel insights into their functional mechanisms. He has received several awards, most recently the JSPS Prize (2018). From 2019, he is an elected board member of the Biophysical Society of Japan.

 

        invited speaker: Florence TamaNagoya University & RIKEN, Japan  

 

 

Florence Tama is a Professor in the Department of Physics and the Institute of Transformative biomolecules at Nagoya University and a team leader at the Center for Computational Science in RIKEN. She studied Physics and Chemistry at Paul Sabatier University in Toulouse and received her Ph.D. in Biophysics in 2000. During her post-doctoral research at the Scripps Research Institute, San Diego, she started to work on the development of computational tools to integrate X-ray and cryo-EM data to build 3D models of biomolecules. In 2006, she joined the University of Arizona as an assistant professor, where she continued such developments with applications to SAXS data. In 2013, she moved to Japan, first to RIKEN, and since 2015 Nagoya University. In recent years, she has extended her work on the development of integrative modeling to X-ray Free Electron Laser data and high-speed AFM data and worked in collaboration with experimental groups to elucidate the function of biomolecules.

Lab website: https://sites.google.com/view/computationalbiophysicslab