AFM BioMed Conference


  DAY 1 | 11 APRIL 2016  

13:30 Welcome

13:45 Introduction

14:00 High-resolution imaging of biological sites using PeakForce QNM
Alexander Dulebo, Bruker Nano Surfaces

14:20 Understanding cells mechanical properties using Atomic Force Microscopy
Samuel Lesko, Bruker Nano Surfaces

14:40 Q&A

15:00 Coffee Break

(1 session to be selected between 3 choices available)
1. Dynamics of DNA Self Assembly using Dimension FastScan Bio
2. Mechanobiology of Cells and Biomaterialsusing Bioscope Resolve
3. Topography screening with higher frequencies PeakForce Tapping using MultiMode

16:00 Break and group switch

(1 session to be selected between 3 choices available)
1. Dynamics of DNA Self Assembly using Dimension FastScan Bio
2. Mechanobiology of Cells and Biomaterialsusing Bioscope Resolve
3. Topography screening with higher frequencies PeakForce Tapping using MultiMode

18:30 Additional demonstrations if needed

19:00 End


  DAY 2 | 12 APRIL 2016  


08:30 – 9:00 Registration / Poster Installation

09:00 – 9:20 Welcome


Topic: Health and Disease

09:20 – 10:00 The Dubrovnik Procedure: An european approach to standardize cell elasticity measurements
Hermann Schillers | Institute of Physiology II, University Münster, Germany


Analysis of the Effect of LRP-1 Silencing on the Invasive Potential of Cancer Cells by Nanomechanical Probing and Adhesion Force Measurements using Atomic Force Microscopy
Anthony Le Cigne | LRN EA4682– Laboratoire de Recherche en Nanosciences, Université de Reims Champagne Ardenne, France

Single Molecule Force Spectroscopy Applied to Heparin-Induced Thrombocytopenia
Thi-Huong Nguyen
| Institut für Immunologie und Transfusionsmedizin/ 2ZIK HIKE - Center for Innovation Competence, Humoral Immune Reactions in Cardiovascular Diseases, Universitätsmedizin Greifswald, Germany

The tumor microenvironment modulates the strength of cell-cell contacts in human melanoma
Verena Hofschröer
| Institute of Physiology II, University of Münster, Germany ´


11:00 – 11:20 Coffee break


11:20 – 11:50 Stiffer Intercalated Discs and Thicker Extracellular Matrix Maintains Contractile Function of the Aged Heart
Adam Engler | Department of Bioengineering and Biomedical Sciences Program, University of California / Sanford Consortium for Regenerative Medicine, USA



Anticancer Drugs Increase Disorder And Reduces Complexity In The Macrophage Membrane
Arkady Bitler | Physics Department and Institute of Nanotechnology, Bar Ilan University / Department of Chemical Research Support, Faculty of Chemistry, Weizmann Institute of Science, Israel

A new therapeutical approach against Pseudomonas aeruginosa: the LecA lectin/galactoclusters affinity studied by Single Molecule Force Spectroscopy
Magali Phaner-Goutorbe | INL- Institut des Nanotechnologies de Lyon (UMR CNRS 5270), Ecole Centrale de Lyon, Université de Lyon, France


12:30 – 14:00 Lunch and Poster Session I


Topic: Mechanobiology

14:00 – 14:30 Are Cancer Cells different? Insights from visco-elastic creep response
Manfred Radmacher | Professor (Full) Universität Bremen, Bremen · Institute of Biophysics, Germany


How endothelial cells can feel hydrostatic pressure
Valeria Prystopiuk | Institute of Physiology II, University of Münster, Germany

Measuring viscoelastic parameters of cells directly from atomic force microscopy force-distance curves
Yuri M. Efremov | Birck Nanotechnology Center / School of Mechanical Engineering, Purdue University, USA

Stress-strain curve of a single antibody in liquid
Alma P. Perrino | Instituto de Ciencia de Materiales de Madrid, Spain

15:30 – 16:00 Probing mechanical properties of the extracellular matrix with AFM
Daniel Navajas | Institute for Bioengineering of Catalonia (IBEC)/ School of Medicine, University of Barcelona/ CIBER of Respiratory Diseases, Spain


16:00 – 16:20 Coffee break



Biomechanical analysis of breast cancer cells to study P-cadherin/SFK mechanotransduction signalling
Filomena A. Carvalho | Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Portugal

Morphology and Nanomechanical Properties of Murine Isolated Liver Sinusoidal Endothelial Cells
Bartlomiej Zapotoczny | Centre for Nanometer-Scale Science and Advanced Materials, NANOSAM, Faculty of Physics, Astronomy, and Applied Computer Science, Jagiellonian University, Poland

Quantitating membrane bleb stiffness using AFM force spectroscopy and an optical sideview setup
Clemens M. Franz | Center for Functional Nanostructures, Karlsruhe Institut of Technology, Germany


20:00 – 23:00 Welcome Dinner by Bruker at Árvore Restaurant 


  DAY 3 | 13 APRIL 2016  


Topic: Nanomedicine

09:00 – 09:40 Realising the impact of SPM in pharmaceutical science and industry
Phil Williams | Laboratory of Biophysics and Surface Analysis, School of Pharmacy, University of Nottingham, UK


AFM study of diamond particles internalization by monitoring MCF7 cell membrane stiffness changes
Marta Martin-Fernandez | Laboratoire Charles Coulomb (L2C), UMR 5221 CNRS-Université de Montpellier, France

Crowding redefines Endonuclease Recognition in Highly Dense DNA Nanoreactors
Abimbola Feyisara Adedeji | Dept. of Physics, University of Trieste/ Dept. of Medical and Biological Sciences, University of Udine, Italy

Fluorescence Tracking of Genome Release during Mechanical Unpacking of Single Viruses
Pedro J. de Pablo | Departamento de Física de la Materia Condensada, Universidad Autónoma de Madrid, Spain


10:40 – 11:10 Coffee break


11:10 – 11:40 High-Speed Atomic Force Microscopy: The dawn of dynamic structural biochemistry
Simon Scheuring | INSERM / Aix-Marseille Université, France



Structural insights into translation repression via AFM imaging of mRNA/protein complexes
Loic Hamon | Laboratoire Structure-Activité des Biomolécules Normales et Pathologiques, INSERM U1204 and Université d’Evry, Université Paris-Saclay, France

Proteins sense different grain orientations in hydroxyapatite during adsorption
Thomas Faidt | Department of Experimental Physics, Saarland University, Germany


12:20 – 12:30 Group Photo

12:30 – 14:00 Lunch and Poster Session II


Topic: Health and Disease

14:00 – 14:30 Atomic force microscopy as a tool to evaluate the risk for cardiovascular diseases in patients
Nuno C. Santos | Faculdade de Medicina da Universidade de Lisboa, iMM Lisboa, Portugal


Probing mechanical properties of young and senescent human fibroblasts: a critical comparison of Force Volume and Peak Force QNM
Simone Bovio
| Cellular Microbiology and Physics of Infection Group, Center of Infection and Immunity of Lille - Institut Pasteur, CNRS UMR8204, INSERM U1019, regional Hosp. Center of Lille, University of Lille, France

Detection of endothelial dysfunction in ex vivo vessels from ApoE/LDLR-/- mice: A multimodal approach
Martina Maase
| Institute of Physiology II, University of Münster, Germany

Early Impairment Of Lung Mechanics In A Murine Model Of Marfan Syndrome
Juan Jose Uriarte
| Unitat Biofísica i Bioenginyeria, Universitat de Barcelona / Institute for Bioengineering of Catalonia / CIBER de Enfermedades Respiratorias, Spain


15:30 – 16:00 Coffee break



Biomolecular mechanisms in Alzheimer’s disease: investigation by high speed Atomic Force Microscopy and Infrared spectroscopy of the interaction between toxic amyloid peptides and model membranes
Michael Molinari | LRN EA4682– Laboratoire de Recherche en Nanosciences, Université de Reims Champagne Ardenne, France

Single molecule force spectroscopy study for curli-mediate bacterial adhesion
Yoojin Oh | Institute of Biophysics, Johannes Kepler University Linz, Austria

The impact of hyperglycemia on adhesion between endothelial and cancer cells
Katarzyna Malek-Zietek | Centre for Nanometer-Scale Science and Advanced Materials, NANOSAM, Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, Krakow, Poland


 DAY 4 | 14 APRIL 2016   


Topic: Mechanobiology

9:00 – 09:40 Elasticity as a cellular response to changing properties of surrounding microenvironment.
Malgorzata Lekka | Institute of Nuclear Physics, Polish Academy of Sciences, Poland


A Microfluidic Chip To Measure The Effect Of Oxygen On Cell Mechanics Probed With AFM
Ignasi Jorba
| Unitat de Biofísica i Bioenginyeria, Universitat de Barcelona / Institute for Bioengineering of Catalonia, Spain

Mapping the viscoelastic creep behavior of cancer cells by atomic force microscopy
Nicolas Schierbaum
| IAP - Institute of Applied Physics, University of Tübingen, Germany

Towards higher-throughput platforms for cell mechanobiology
Sanjay Kumar
| Department of Bioengineering, Department of Chemical & Biomolecular Engineering, University of California, US


10:40 – 11:10 Coffee break



Quantitative measurements of the viscosity of biomimetic membranes using the AFM circular mode
Sebastian Jaramillo Isaza | Roberval CNRS, UMR 7337 - Sorbonne university, Université de technologie de Compiègne, France

High-Resolution Studies of Cell Structure and Mechanics by Atomic Force Microscopy
Alexander Dulebo | Bruker Nano Surfaces, USA


12:00 – 14:00 Lunch and Poster Session III


Topic: Bioimaging

14:00 – 14:30 Real-time visualisation of membrane pore formation by bacterial toxins
Bart Hoogenboom | London Centre for Nanotechnology, University College London, UK



Simultaneous advanced microscopies for live cell signaling dynamics investigations
Pieter A. A. De Beule | International Iberian Nanotechnology Laboratory, Portugal

Force-controlled Patch Clamp and Scanning Ion Conductance Microscopy with the FluidFM
Livie Dorwling-Carter | LBB – Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zürich, Switzerland

Revealing contact formation characteristics of bacteria
Karin Jacobs | Experimental Physics, Saarland University, Germany


15:30 – 16:00 Coffee break



Sphingomyelin-rich domains in bilayer models of the milk fat globule membrane: temperature governs structural and mechanical heterogeneity
Fanny Guyomarc’h | INRA / AGROCAMPUS OUEST UMR 1253, Science and Technology of Milk and Egg, France

Nanoscale Infrared Spectroscopy and functional imaging of proteins
Andrzej Kulik | Physics of Living Matter, EPFL, Switzerland

Real-time interactions of pharmaceutical compounds with lipid rafts: from nanoscale lipid organization to immunosensing
Joaquim T. Marques | Centro de Química e Bioquímica. Faculdade de Ciências, Universidade de Lisboa, Portugal


17:30 Departure from the Biblioteca Almeida Garrett to the social programme

18:00 – 20:00 Visit to Taylor’s Port Wine Cellars

20:00 – 23:00 Dinner at Taylor’s Port Wine Cellars


  DAY 5 | 15 APRIL 2016  


Topic: Bioimaging

09:00 - 09:40 Recognition force microscopy and high-speed AFM
Peter Hinterdorfer
| Johannes Kepler University, Institute for Biophysics, Austria



The Architecture of Neutrophil Extracellular Traps revealed by AFM Imaging and Force Spectroscopy
Ricardo H. Pires | ZIK-HIKE, University of Greifswald/ Department of Internal Medicine B, Cardiology, University Medicine Greifswald, Germany / DZHK (German Centre for Cardiovascular Research), partner site Greifswald, Germany

Correlated atomic force microscopy and single molecule localization microscopy
Pascal D. Odermatt | Laboratory for Bio- and Nano-Instrumentation, Institute of Bioengineering, School of Engineering, EPFL, Switzerland

Resolving the division process in Mycobacterium Smegmatis; from milliseconds to days
Georg E. Fantner | Interfaculty Institute for Bioengineering – Laboratory for Bio and Nanoinstrumentation, École Polytechnique Fédéral de Lausanne, Switzerland


10:40 – 11:10 Coffee break


Local hydration properties of single bacterial cells and spores by lift-mode Electrostatic Force Microscopy
Marc Van Der Hofstadt | IBEC – Institut de Bioenginyeria de Catalunya, Spain

The organization of membrane proteins in rod outer segments revealed by AFM imaging and SMFS
Sourav Maity | International School for Advanced Studies (SISSA-ISAS), Italy

ESCRT-III spiral springs: polymerization and depolymerization observed by High-Speed AFM
Lorena Redondo-Morata | 1U1006 INSERM, Aix-Marseille Université, France

Fluctuating Lipid Nanodomains Near Critical Transitions
Simon D. Connell | School of Physics and Astronomy, University of Leeds, United Kingdom

12:30 – 14:00 Lunch and Poster Session IV


Topic: Nanomedicine

14:00 – 14:30 Nanotechnology at the service of nerve regeneration
Ana Paula Pêgo | NanoBiomaterials for Targeted Therapies i3S/INEB, Portugal



On chip biodetection, analysis and nanometrology of blood microparticles
Celine Elie-Caille | FEMTO-ST Institute, UFC, CNRS, ENSMM, UTBM, UBFC, France

Quantification of tumor cell–platelet adhesion by single-cell force spectroscopy to: a tool to identify new targets for cancer therapies
Hermann Schillers |  Institute of Physiology II, University of Münster, Germany

The conformational equilibria of the prion protein characterized by AFM-based single-molecule force spectroscopy
Giampaolo Zuccheri | Department of Pharmacy and Biotechnologies, University of Bologna/ S3 Center, Institute of Nanoscience of the Italian CNR, Italy

Cardiomyocyte syncytium combined with Atomic force microscopy, advanced setup of universal biosensor for phenotype screening
Martin Pesl | International Clinical Research Center, St. Anne’s University Hospital / Department of Biology, Faculty of Medicine, Masaryk University, Czech Republic


15:30 – 16:00 Coffee break

16:00 – 16:20 Awards and Closing Session